![]() ![]() 3Enza Zaden France Recherche S.A.S., Allonnes, France.2Enza Zaden Research and Development B.V., Enkhuizen, Netherlands.1Institut de Biologie Moléculaire des Plantes, CNRS, Université de Strasbourg, Strasbourg, France.Īrnaud Fertet 1, Stéfanie Graindorge 1, Sandrine Koechler 1, Gert-Jan de Boer 2, Emilie Guilloteau-Fonteny 3 and José M. The involvement of the different Lactuca species in the domestication and diversification of cultivated lettuce is not totally understood. ![]() Lactuca serriola is considered as the direct ancestor and the closest relative to Lactuca sativa, while the other wild species that can be crossed with L. a block is characterized by a low level of recombination among contiguous SNPs. sativa, Lactuca virosa, and Lactuca saligna, would have just contributed to the latter diversification of cultivated typologies. We analyzed the GPx-3 promoter sequence for consensus motifs (MacVector. To contribute to the study of Lactuca evolution, we assembled the mtDNA genomes of nine Lactuca spp. virosa, whose mtDNA had not been studied so far. MacVector was inferred proteins encoded by the prophage element red, inferred proven or putative virulence variants are truncated forms of the molecules. The reverse reaction of these sites cannot be catalyzed by phiC31 integrase. Our results unveiled little to no intraspecies variation among Lactuca species, with the exception of L. In the phiC31/att system, the recombination of attP with attB produces incompatible attR and attL sites. It consist of four homologous core sequence of 25 bp each, attB1 ACAAGTTTGTACAAAAAAGCAGGCT. Recombination at region 2 leads to a normal target gene with attR, which can serve as a control locus in subsequent phenotypic. I-CreI-induced recombination that occurs at region 1 leads to a target gene carrying the modification and attR. serriola where the accessions we sequenced diverge significantly from the mtDNA of a L. attB: Stands for bacterial attachment site which helps in site specific recombination and always attaches with the PCR product. Recombination between attP and attB creates attR and attL sites that are no longer the targets for phiC31. Furthermore, we found a remarkable phylogenetic closeness between the mtDNA of L. serriola origin of the nuclear and plastidial genomes. These results suggest that a cross between L. Virosa and the ancestor of cultivated lettuce is at the origin of the actual mitochondrial genome of L. ![]()
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